| UniProt/SwissProt ID: | CHK1_HUMAN |
| Description: | checkpoint kinase 1 [Source:HGNC Symbol;Acc:1925] |
| Location: | chr11 q24.2 |
| Node attribute: | PPI(source); substrate; kinase; gene |
| Phosphorylation site |
| Position | PhosphoPeptide | Catalytic kinase | Source |
|---|---|---|---|
| S317 | VKYSSsQPEPR | PhosphoSitePlus; phosphoELM; SysPTM | |
| S280 | RPRVTsGGVSE | AKT1(PKB) | RegPhos |
| S280 | RPRVTsGGVSE | AKT1 | HPRD; RegPhos |
| S345 | QGISFsQPTCP | ATR | HPRD; phosphoELM; RegPhos |
| S280 | RPRVTsGGVSE | PhosphoSitePlus; phosphoELM | |
| S345 | QGISFsQPTCP | PhosphoSitePlus; phosphoELM | |
| S305 | FSPVNsASSEE | PhosphoSitePlus; HPRD | |
| T362 | SQLLGtPGSSQ | PhosphoSitePlus | |
| S280 | RPRVTsGGVSE | PKB_group | phosphoELM |
| S343 | LVQGIsFSQPT | PhosphoSitePlus | |
| T330 | LSLWDtSPSYI | PhosphoSitePlus; HPRD | |
| T348 | SFSQPtCPDHM | PhosphoSitePlus | |
| S365 | LGTPGsSQNPW | PhosphoSitePlus | |
| S291 | SPSGFsKHIQS | PhosphoSitePlus | |
| S301 | SNLDFsPVNSA | PhosphoSitePlus; HPRD; phosphoELM; SysPTM | |
| S284 | TSGGVsESPSG | PhosphoSitePlus; HPRD; phosphoELM | |
| S333 | WDTSPsYIDKL | PhosphoSitePlus | |
| S366 | GTPGSsQNPWQ | PhosphoSitePlus | |
| S301 | SNLDFsPVNSA | CDK1 | phosphoELM; SysPTM |
| S467 | LIDIVsSQKIW | PhosphoSitePlus | |
| S307 | PVNSAsSEENV | HPRD | |
| S468 | IDIVSsQKIWL | PhosphoSitePlus; HPRD; SysPTM | |
| S296 | SKHIQsNLDFS | PhosphoSitePlus; HPRD; phosphoELM; RegPhos | |
| S357 | HMLLNsQLLGT | PhosphoSitePlus | |
| S308 | VNSASsEENVK | PhosphoSitePlus; HPRD; phosphoELM; RegPhos; SysPTM | |
| S286 | GGVSEsPSGFS | PhosphoSitePlus; HPRD; phosphoELM; SysPTM | |
| S315 | ENVKYsSSQPE | PhosphoSitePlus | |
| S331 | SLWDTsPSYID | PhosphoSitePlus; HPRD; phosphoELM; SysPTM | |
| S345 | QGISFsQPTCP | ATM | phosphoELM; RegPhos |
| S280 | RPRVTsGGVSE | AKT_group | RegPhos |
| T279 | KRPRVtSGGVS | PhosphoSitePlus; HPRD | |
| S316 | NVKYSsSQPEP | PhosphoSitePlus | |
| S288 | VSESPsGFSKH | PhosphoSitePlus; HPRD | |
| S389 | LDADKsYQCLK | PhosphoSitePlus | |
| S317 | VKYSSsQPEPR | ATR | HPRD; phosphoELM; RegPhos; SysPTM |
| S317 | VKYSSsQPEPR | ATM | phosphoELM; RegPhos; SysPTM |
| Dephosphorylation site |
| CHK1_HUMAN do not have dephosphorylation site. |
| Mutation site |
| Ensembl ID | Variation | Source | Cancer name | Pubmed |
|---|---|---|---|---|
| ENSP00000388648 | E320K | BIOMART | Neoplasms by Histologic Type | 21930507, 21930502 | ENSP00000388648 | R160H | TCGA | Neoplasms by Histologic Type | 21720365, 18772890 | ENSP00000432890 | R160H | TCGA | Neoplasms by Histologic Type | 21720365, 18772890 | ENSP00000412504 | R160H | TCGA | Neoplasms by Histologic Type | 21720365, 18772890 | ENSP00000435371 | R160H | TCGA | Neoplasms by Histologic Type | 21720365, 18772890 |
| Differential expressed protein |
| CHK1_HUMAN is not differential expressed protein. |
| Hyperphosphorylation site |
| Site | Motif | Peptide | Cancer name | Pubmed | Source |
|---|---|---|---|---|---|
| S280 | AKRPRVTsGGVSESP | breast cancer | 17418864 | PhosphoSitePlus | S345 | LVQGISFsQPTCPDH | RIDDLE syndrome | 17940005 | PhosphoSitePlus |
| Direct Interaction Pair |
| Source | Target | Relationship | Resource |
|---|---|---|---|
| CHK1_HUMAN | MCM4_HUMAN | kinase -> substrate | RegPhos |
| CHK1_HUMAN | MDM4_HUMAN | kinase -> substrate | HPRD |
| CHK1_HUMAN | RAD51_HUMAN | kinase -> substrate | HPRD; RegPhos |
| CHK1_HUMAN | CLSPN_HUMAN | kinase -> substrate | HPRD; RegPhos |
| ZAGL2_HUMAN | CHK1_HUMAN | kinase -> substrate | RegPhos |
| MAPK5_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| ATM_HUMAN | CHK1_HUMAN | kinase -> substrate | RegPhos |
| MK13_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| DDR1_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| CHK1_HUMAN | P73_HUMAN | kinase -> substrate | RegPhos |
| CHK1_HUMAN | MPIP1_HUMAN | kinase -> substrate | RegPhos |
| ILK_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| IRAK1_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| AKT1_HUMAN | CHK1_HUMAN | kinase -> substrate | HPRD; RegPhos |
| TESK2_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| HIPK1_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| ACK1_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| CHK1_HUMAN | CDK1_HUMAN | kinase -> substrate | KEGG |
| CHK1_HUMAN | MPIP3_HUMAN | kinase -> substrate | HPRD; RegPhos |
| CDK3_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| CHK1_HUMAN | P53_HUMAN | kinase -> substrate | RegPhos |
| CHK1_HUMAN | TLK1_HUMAN | kinase -> substrate | HPRD; RegPhos |
| ATR_HUMAN | CHK1_HUMAN | kinase -> substrate | RegPhos |
| NUAK1_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| M3K8_HUMAN | CHK1_HUMAN | kinase -> substrate | Robert H Newman (2013) |
| CHK1_HUMAN | ESPL1_HUMAN | other | KEGG |
| P53_HUMAN | CHK1_HUMAN | TF -> target gene | TRANSFAC |
| Function Annotation |
| KEGG Pathway |
| KEGG ID | Pathway |
|---|---|
| hsa05166 | HTLV-I infection |
| hsa04110 | Cell cycle |
| hsa05203 | Viral carcinogenesis |
| hsa04115 | p53 signaling pathway |
| Gene Ontology |
| GO ID | GO_Term | Evidence | Ontology |
|---|---|---|---|
| GO:0035402 | histone kinase activity (H3-T11 specific) | IEA | molecular_function |
| GO:0000785 | chromatin | IEA | cellular_component |
| GO:0006260 | DNA replication | TAS | biological_process |
| GO:0005813 | centrosome | IDA | cellular_component |
| GO:0071313 | cellular response to caffeine | IEA | biological_process |
| GO:0004672 | protein kinase activity | IEA | molecular_function |
| GO:0071260 | cellular response to mechanical stimulus | IEP | biological_process |
| GO:0000794 | condensed nuclear chromosome | IDA | cellular_component |
| GO:0000077 | DNA damage checkpoint | IDA | biological_process |
| GO:0042127 | regulation of cell proliferation | IEA | biological_process |
| GO:0005515 | protein binding | IEA | molecular_function |
| GO:0045839 | negative regulation of mitosis | IDA | biological_process |
| GO:2000615 | regulation of histone H3-K9 acetylation | IEA | biological_process |
| GO:0018107 | peptidyl-threonine phosphorylation | IDA | biological_process |
| GO:0005829 | cytosol | TAS | cellular_component |
| GO:0006468 | protein phosphorylation | IEA | biological_process |
| GO:0033261 | regulation of S phase | IEA | biological_process |
| GO:0005515 | protein binding | IPI | molecular_function |
| GO:0035407 | histone H3-T11 phosphorylation | IDA | biological_process |
| GO:0000075 | cell cycle checkpoint | TAS | biological_process |
| GO:0005634 | nucleus | IEA | cellular_component |
| GO:0000086 | G2/M transition of mitotic cell cycle | IEA | biological_process |
| GO:0031572 | G2/M transition DNA damage checkpoint | IEA | biological_process |
| GO:0005657 | replication fork | IEA | cellular_component |
| GO:0090399 | replicative senescence | NAS | biological_process |
| GO:0010767 | regulation of transcription from RNA polymerase II | IEA | biological_process |
| GO:0006975 | DNA damage induced protein phosphorylation | IEA | biological_process |
| GO:0016772 | transferase activity, transferring phosphorus-cont | IEA | molecular_function |
| GO:0010569 | regulation of double-strand break repair via homol | IDA | biological_process |
| GO:0010468 | regulation of gene expression | IEA | biological_process |
| GO:0048096 | chromatin-mediated maintenance of transcription | IEA | biological_process |
| GO:0006281 | DNA repair | IEA | biological_process |
| GO:0000781 | chromosome, telomeric region | IDA | cellular_component |
| GO:0006974 | response to DNA damage stimulus | IEA | biological_process |
| GO:0005654 | nucleoplasm | TAS | cellular_component |
| GO:0004674 | protein serine/threonine kinase activity | IDA | molecular_function |
| GO:0006974 | response to DNA damage stimulus | IMP | biological_process |
| GO:0004713 | protein tyrosine kinase activity | IEA | molecular_function |
| GO:0005524 | ATP binding | IEA | molecular_function |
| GO:0071310 | cellular response to organic substance | IEA | biological_process |
| GO:0046602 | regulation of mitotic centrosome separation | IDA | biological_process |
| GO:0000077 | DNA damage checkpoint | IEA | biological_process |