Phosphorylation

Protein information

Protein summary

Phosphorylation site

Dephosphorylation site

Mutation site

Differential expressed protein

Hyperphosphorylation site

Direct interaction pair

Function Annotation

Protein Summary

UniProt/SwissProt ID:	TNFA_HUMAN
Description:	tumor necrosis factor [Source:HGNC Symbol;Acc:11892]
Location:	chrHSCHR6_MHC_DBB p2
Node attribute:	PPI(target); PPI(source); phosphotase; substrate; ligand; gene

Phosphorylation site

Position	PhosphoPeptide	Catalytic kinase	Source
S2	MSTESmI	CK1_group	RegPhos
S2	ESMI	CK2_group	phosphoELM
S2	ESMI	PDIK1L	HPRD
S2	MSTESmI	CK2_group	RegPhos
S162	SRIAVsYQTKV	TNF	PhosphoSitePlus
S2	MSTESmI	CLIK1L	RegPhos
S171	KVNLLsAIKSP	TNF	PhosphoSitePlus

Dephosphorylation site

TNFA_HUMAN do not have dephosphorylation site.

Mutation site

Ensembl ID	Variation	Source	Cancer name	Pubmed
ENSP00000398698	P146L	COSMIC	Melanoma
ENSP00000398698	L151F	COSMIC	Ovarian Cancer	21720365
ENSP00000398698	R207*	COSMIC	Pancreatic Cancer

Differential expressed protein

TNFA_HUMAN is not differential expressed protein.

Hyperphosphorylation site

TNFA_HUMAN do not have hyperphosphorylation site.

Direct Interaction Pair

Source	Target	Relationship	Resource
TNFA_HUMAN	TNR21_HUMAN	ligand -> receptor	HPMR
TNFA_HUMAN	TNR1B_HUMAN	ligand -> receptor	HPMR
TNFA_HUMAN	TNR1A_HUMAN	ligand -> receptor	HPMR
GRK5_HUMAN	TNFA_HUMAN	kinase -> substrate	Robert H Newman (2013)
PDK1L_HUMAN	TNFA_HUMAN	kinase -> substrate	HPRD; RegPhos
TNFA_HUMAN	BCL2_HUMAN	phosphatase -> substrate	HPRD
TNFA_HUMAN	TNR1A_HUMAN	other	KEGG
NFKB1_HUMAN	TNFA_HUMAN	other	KEGG
FOS_HUMAN	TNFA_HUMAN	other	KEGG
TNFA_HUMAN	SMAD6_HUMAN	other	KEGG
TNFA_HUMAN	IKKB_HUMAN	other	KEGG
TNFA_HUMAN	TNR1B_HUMAN	other	KEGG
IRF5_HUMAN	TNFA_HUMAN	TF -> target gene	KEGG
LITAF_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
SP1_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
EGR1_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
JUN_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
ETS1_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
REL_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
ATF2_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
TF65_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC
ELK1_HUMAN	TNFA_HUMAN	TF -> target gene	TRANSFAC

Function Annotation

KEGG Pathway

KEGG ID	Pathway
hsa04010	MAPK signaling pathway
hsa04930	Type II diabetes mellitus
hsa04940	Type I diabetes mellitus
hsa04622	RIG-I-like receptor signaling pathway
hsa05330	Allograft rejection
hsa05166	HTLV-I infection
hsa04620	Toll-like receptor signaling pathway
hsa05414	Dilated cardiomyopathy
hsa05410	Hypertrophic cardiomyopathy (HCM)
hsa04210	Apoptosis
hsa04650	Natural killer cell mediated cytotoxicity
hsa04621	NOD-like receptor signaling pathway
hsa05168	Herpes simplex infection
hsa05332	Graft-versus-host disease
hsa04612	Antigen processing and presentation
hsa05133	Pertussis
hsa04350	TGF-beta signaling pathway
hsa04150	mTOR signaling pathway
hsa05322	Systemic lupus erythematosus
hsa04380	Osteoclast differentiation
hsa04640	Hematopoietic cell lineage
hsa05010	Alzheimer's disease
hsa05140	Leishmaniasis
hsa04664	Fc epsilon RI signaling pathway
hsa05146	Amoebiasis
hsa05145	Toxoplasmosis
hsa05310	Asthma
hsa05160	Hepatitis C
hsa05134	Legionellosis
hsa05014	Amyotrophic lateral sclerosis (ALS)
hsa04920	Adipocytokine signaling pathway
hsa04060	Cytokine-cytokine receptor interaction
hsa05144	Malaria
hsa05143	African trypanosomiasis
hsa05323	Rheumatoid arthritis
hsa04660	T cell receptor signaling pathway
hsa05152	Tuberculosis
hsa04064	NF-kappa B signaling pathway
hsa05164	Influenza A
hsa05142	Chagas disease (American trypanosomiasis)

Gene Ontology

GO ID	GO_Term	Evidence	Ontology
GO:0060557	positive regulation of vitamin D biosynthetic proc	IDA	biological_process
GO:0006919	activation of cysteine-type endopeptidase activity	IDA	biological_process
GO:0001891	phagocytic cup	IEA	cellular_component
GO:0061048	negative regulation of branching involved in lung	IDA	biological_process
GO:0032722	positive regulation of chemokine production	IDA	biological_process
GO:0030198	extracellular matrix organization	IEA	biological_process
GO:0050715	positive regulation of cytokine secretion	IDA	biological_process
GO:0071407	cellular response to organic cyclic compound	IDA	biological_process
GO:0007254	JNK cascade	IEA	biological_process
GO:0008625	extrinsic apoptotic signaling pathway via death do	IEA	biological_process
GO:0060555	activation of necroptosis by extracellular signals	IDA	biological_process
GO:0050900	leukocyte migration	IEA	biological_process
GO:0032755	positive regulation of interleukin-6 production	IEA	biological_process
GO:0006927	transformed cell apoptotic process	IDA	biological_process
GO:0045892	negative regulation of transcription, DNA-dependen	IDA	biological_process
GO:0045893	positive regulation of transcription, DNA-dependen	IEA	biological_process
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB c	IEA	biological_process
GO:0045893	positive regulation of transcription, DNA-dependen	IDA	biological_process
GO:0060559	positive regulation of calcidiol 1-monooxygenase a	IDA	biological_process
GO:0006955	immune response	IEA	biological_process
GO:0042127	regulation of cell proliferation	IEA	biological_process
GO:0050708	regulation of protein secretion	IEA	biological_process
GO:0097190	apoptotic signaling pathway	IDA	biological_process
GO:0005125	cytokine activity	IEA	molecular_function
GO:0000165	MAPK cascade	IMP	biological_process
GO:0006954	inflammatory response	IDA	biological_process
GO:0045994	positive regulation of translational initiation by	IEA	biological_process
GO:0002439	chronic inflammatory response to antigenic stimulu	IMP	biological_process
GO:0009615	response to virus	IDA	biological_process
GO:0006952	defense response	IEA	biological_process
GO:0001819	positive regulation of cytokine production	IDA	biological_process
GO:0010629	negative regulation of gene expression	IDA	biological_process
GO:0006959	humoral immune response	IEA	biological_process
GO:0031622	positive regulation of fever generation	IEA	biological_process
GO:0042802	identical protein binding	IDA	molecular_function
GO:0042742	defense response to bacterium	IEA	biological_process
GO:0001666	response to hypoxia	IEA	biological_process
GO:0045840	positive regulation of mitosis	IEA	biological_process
GO:0050796	regulation of insulin secretion	IDA	biological_process
GO:0010888	negative regulation of lipid storage	NAS	biological_process
GO:0006915	apoptotic process	TAS	biological_process
GO:0031334	positive regulation of protein complex assembly	IDA	biological_process
GO:0006917	induction of apoptosis	IEA	biological_process
GO:0016021	integral to membrane	IEA	cellular_component
GO:0045944	positive regulation of transcription from RNA poly	IEA	biological_process
GO:0051798	positive regulation of hair follicle development	IEA	biological_process
GO:0009887	organ morphogenesis	IEA	biological_process
GO:0008285	negative regulation of cell proliferation	IEA	biological_process
GO:0005164	tumor necrosis factor receptor binding	IEA	molecular_function
GO:0005886	plasma membrane	TAS	cellular_component
GO:0045668	negative regulation of osteoblast differentiation	IEA	biological_process
GO:0071677	positive regulation of mononuclear cell migration	NAS	biological_process
GO:0043068	positive regulation of programmed cell death	IDA	biological_process
GO:0010940	positive regulation of necrotic cell death	TAS	biological_process
GO:2000010	positive regulation of protein localization to cel	IDA	biological_process
GO:0045429	positive regulation of nitric oxide biosynthetic p	IDA	biological_process
GO:0046330	positive regulation of JNK cascade	IEA	biological_process
GO:0001932	regulation of protein phosphorylation	IEA	biological_process
GO:0043406	positive regulation of MAP kinase activity	IDA	biological_process
GO:0051091	positive regulation of sequence-specific DNA bindi	IDA	biological_process
GO:0045123	cellular extravasation	IEA	biological_process
GO:0009612	response to mechanical stimulus	IEA	biological_process
GO:0005887	integral to plasma membrane	IDA	cellular_component
GO:0006915	apoptotic process	IEA	biological_process
GO:0005515	protein binding	IEA	molecular_function
GO:0003009	skeletal muscle contraction	IEA	biological_process
GO:0045080	positive regulation of chemokine biosynthetic proc	IDA	biological_process
GO:0050830	defense response to Gram-positive bacterium	IEA	biological_process
GO:0000060	protein import into nucleus, translocation	IDA	biological_process
GO:0001775	cell activation	IEA	biological_process
GO:0016020	membrane	IEA	cellular_component
GO:0043525	positive regulation of neuron apoptotic process	IEA	biological_process
GO:0019722	calcium-mediated signaling	IEA	biological_process
GO:0050995	negative regulation of lipid catabolic process	IDA	biological_process
GO:0033138	positive regulation of peptidyl-serine phosphoryla	IDA	biological_process
GO:0050901	leukocyte tethering or rolling	IDA	biological_process
GO:0007165	signal transduction	IEA	biological_process
GO:0002740	negative regulation of cytokine secretion involved	IDA	biological_process
GO:0002876	positive regulation of chronic inflammatory respon	IEA	biological_process
GO:0032715	negative regulation of interleukin-6 production	IDA	biological_process
GO:0030730	sequestering of triglyceride	IDA	biological_process
GO:0030316	osteoclast differentiation	IEA	biological_process
GO:0051222	positive regulation of protein transport	IDA	biological_process
GO:0045121	membrane raft	IEA	cellular_component
GO:0051092	positive regulation of NF-kappaB transcription fac	IDA	biological_process
GO:0000122	negative regulation of transcription from RNA poly	IEA	biological_process
GO:2000343	positive regulation of chemokine (C-X-C motif) lig	IDA	biological_process
GO:0044130	negative regulation of growth of symbiont in host	IEA	biological_process
GO:0005622	intracellular	IEA	cellular_component
GO:0005515	protein binding	IPI	molecular_function
GO:0051897	positive regulation of protein kinase B signaling	IEA	biological_process
GO:0002925	positive regulation of humoral immune response med	IEA	biological_process
GO:0070265	necrotic cell death	IDA	biological_process
GO:0001934	positive regulation of protein phosphorylation	IDA	biological_process
GO:0042493	response to drug	IEA	biological_process
GO:0005886	plasma membrane	IEA	cellular_component
GO:0010693	negative regulation of alkaline phosphatase activi	IEA	biological_process
GO:0045599	negative regulation of fat cell differentiation	NAS	biological_process
GO:0043491	protein kinase B signaling cascade	IMP	biological_process
GO:0014823	response to activity	IEA	biological_process
GO:0071316	cellular response to nicotine	IDA	biological_process
GO:0071803	positive regulation of podosome assembly	IDA	biological_process
GO:0002037	negative regulation of L-glutamate transport	IEA	biological_process
GO:0048661	positive regulation of smooth muscle cell prolifer	IDA	biological_process
GO:0070374	positive regulation of ERK1 and ERK2 cascade	NAS	biological_process
GO:0006006	glucose metabolic process	IEA	biological_process
GO:0051023	regulation of immunoglobulin secretion	IEA	biological_process
GO:0005615	extracellular space	IEA	cellular_component
GO:0045672	positive regulation of osteoclast differentiation	IDA	biological_process
GO:0000185	activation of MAPKKK activity	IDA	biological_process
GO:0045416	positive regulation of interleukin-8 biosynthetic	IDA	biological_process
GO:0050806	positive regulation of synaptic transmission	IEA	biological_process
GO:0045670	regulation of osteoclast differentiation	IEA	biological_process
GO:0097191	extrinsic apoptotic signaling pathway	IDA	biological_process
GO:0032800	receptor biosynthetic process	IDA	biological_process
GO:0060664	epithelial cell proliferation involved in salivary	IEA	biological_process
GO:0032729	positive regulation of interferon-gamma production	IEA	biological_process
GO:0033209	tumor necrosis factor-mediated signaling pathway	IEA	biological_process
GO:0051044	positive regulation of membrane protein ectodomain	IDA	biological_process
GO:0071230	cellular response to amino acid stimulus	IEA	biological_process
GO:0044212	transcription regulatory region DNA binding	IDA	molecular_function
GO:0034116	positive regulation of heterotypic cell-cell adhes	IDA	biological_process
GO:0060693	regulation of branching involved in salivary gland	IEA	biological_process
GO:0009897	external side of plasma membrane	IEA	cellular_component
GO:0043122	regulation of I-kappaB kinase/NF-kappaB cascade	IDA	biological_process
GO:0043066	negative regulation of apoptotic process	IDA	biological_process
GO:0048566	embryonic digestive tract development	IEP	biological_process
GO:0031663	lipopolysaccharide-mediated signaling pathway	IDA	biological_process
GO:0055037	recycling endosome	IEA	cellular_component
GO:0007275	multicellular organismal development	IEA	biological_process
GO:0009651	response to salt stress	TAS	biological_process
GO:0009986	cell surface	IDA	cellular_component
GO:0002020	protease binding	IEA	molecular_function
GO:0000187	activation of MAPK activity	IDA	biological_process
GO:0051533	positive regulation of NFAT protein import into nu	IDA	biological_process
GO:0042346	positive regulation of NF-kappaB import into nucle	IEA	biological_process
GO:0043243	positive regulation of protein complex disassembly	IDA	biological_process
GO:0043065	positive regulation of apoptotic process	IDA	biological_process
GO:0051222	positive regulation of protein transport	IEA	biological_process
GO:0046325	negative regulation of glucose import	IEA	biological_process
GO:0051384	response to glucocorticoid stimulus	IDA	biological_process
GO:0045071	negative regulation of viral genome replication	IDA	biological_process

Human Phosphorylation Mediated
Signaling Transduction Network

PhoSigNet

Human Phosphorylation Mediated Signaling Transduction Network

PhoSigNet

Human Phosphorylation Mediated
Signaling Transduction Network