Protein information
  Protein summary    Phosphorylation site    Dephosphorylation site    Mutation site
  Differential expressed protein    Hyperphosphorylation site    Direct interaction pair    Function Annotation

Protein Summary
UniProt/SwissProt ID:MYLK_HUMAN
Description:myosin light chain kinase [Source:HGNC Symbol;Acc:7590]
Location:chr3 q21.1
Node attribute:substrate; kinase; PPI(target)

Phosphorylation site
PositionPhosphoPeptideCatalytic kinaseSource
S1760IGRLSsMAMISphosphoELM; RegPhos; SysPTM
S1691IGRLSsMAMISHPRD
S1653SGRKSsTGSPTHPRD
Y556GQPIQyARSTCI-set; BiPBP_CPhosphoSitePlus
Y611SRKSEyLLPVAPhosphoSitePlus
S1725KSSTGsPTSPLHPRD
T14GRKSStGSPTSHPRD
S16HISKTsLSVDPGSK_groupRegPhos
S343LQKTSsSITLQRegPhos
S1722SGRKSsTGSPTHPRD
T978PPKPAtPDFRSPhosphoSitePlus
S1768MISGLsGRKSSDUF2656PhosphoSitePlus
S1709IGRLSsMAMISHPRD
S16HISKTsLSVDPMAPK_groupphosphoELM; RegPhos
S1759AIGRLsSMAMIRegPhos
S19TGSPTsPLNAEHPRD
S1760IGRLSsMAMISCAMK2GHPRD; SysPTM
S1779TGSPTsPLNAEHPRD; phosphoELM; SysPTM
T1723GRKSStGSPTSHPRD
T1709STGSPtSPLNAHPRD
S1776KSSTGsPTSPLDUF2656PhosphoSitePlus
S1760IGRLSsMAMISDUF2656PhosphoSitePlus; SysPTM
S515MEVAPsFSSVLI-set; BiPBP_CPhosphoSitePlus
T1778STGSPtSPLNAHPRD
S1760IGRLSsMAMISPAK2HPRD; RegPhos; SysPTM
S18SKTSLsVDPSRPAK1HPRD; RegPhos
Y471DAGSHyLCLLKRegPhos
T88CGIRGtFSLVII-setPhosphoSitePlus
Y792WHAGQyEILLKI-setPhosphoSitePlus
T1654GRKSStGSPTSHPRD
S1652LSGRKsSTGSPHPRD
S16HISKTsLSVDPGSK-3_groupphosphoELM
T1658STGSPtSPLNAHPRD
S16KSSTGsPTSPLHPRD
Y1449EPEVDyRTVTIPhosphoSitePlus
S1728TGSPTsPLNAEHPRD
Y846GGSDRyGSLRPPhosphoSitePlus
S1776KSSTGsPTSPLHPRD; phosphoELM; RegPhos
T1778STGSPtSPLNADUF2656PhosphoSitePlus
Y1835CKIEGyPDPEVI-setPhosphoSitePlus
S1773SGRKSsTGSPTDUF2656PhosphoSitePlus
S1772LSGRKsSTGSPDUF2656PhosphoSitePlus
Y1575SEGVEyIHKQGPkinasePhosphoSitePlus
S1759AIGRLsSMAMIDUF2656PhosphoSitePlus
S1779TGSPTsPLNAEDUF2656PhosphoSitePlus; SysPTM
T1705GRKSStGSPTSHPRD
T1774GRKSStGSPTSHPRD; phosphoELM
Y464GSIEVyEDAGSRegPhos
T1727STGSPtSPLNAHPRD
S13ASSHIsKTSLSPKA_groupphosphoELM; RegPhos
S1659TGSPTsPLNAEHPRD
S1707KSSTGsPTSPLHPRD
S1208SRRPKsSLPPVPAK2HPRD; RegPhos
S13SGRKSsTGSPTHPRD
S1656KSSTGsPTSPLHPRD
S1760IGRLSsMAMISCaMK2gRegPhos; SysPTM
Y104EDRGKyTCEATI-setPhosphoSitePlus
S1640IGRLSsMAMISHPRD
S1093EKRSEsQGTAPPhosphoSitePlus
T109YTCEAtNGSGAI-setPhosphoSitePlus
S12LSGRKsSTGSPHPRD
S1710TGSPTsPLNAEHPRD
S814LMLQNsSARALRegPhos
Y1635PEVINyEPIGYPkinasePhosphoSitePlus
T18STGSPtSPLNAHPRD
S1438DEVEVsDDDEKPhosphoSitePlus; HPRD; phosphoELM; SysPTM
S1772LSGRKsSTGSPHPRD; phosphoELM
T1774GRKSStGSPTSDUF2656PhosphoSitePlus
Y471DAGSHyLCLLKI-setPhosphoSitePlus
Y1410RAINVyGTSEPfn3PhosphoSitePlus
S1369DEVEVsDDDEKHPRD
S1721LSGRKsSTGSPHPRD
S1773SGRKSsTGSPTHPRD
S1208SRRPKsSLPPVphosphoELM; RegPhos
Y464GSIEVyEDAGSI-setPhosphoSitePlus
Y231DDVGVyTCLVVI-setPhosphoSitePlus
S1703LSGRKsSTGSPHPRD
S1704SGRKSsTGSPTHPRD
T118GARQVtVELTVI-setPhosphoSitePlus

Dephosphorylation site
MYLK_HUMAN do not have dephosphorylation site.

Mutation site
Ensembl IDVariationSourceCancer namePubmed
ENSP00000418335P1588LHPIOvarian Cancer17344846
ENSP00000353452P1588LGreenman2007, HPIOvarian Cancer17344846, 18428421

Differential expressed protein
Ensembl IDCancer nameDesignSampleChangeRatioPubmed
ENSP00000353452hepatitis C virusNormal vs. CancertissueUp3.118715028
ENSP00000353452Breast CancerNormal vs. CancertissueDown0.8920739354
ENSP00000353452Breast CancerNormal vs. CancertissueDown0.8920739354

Hyperphosphorylation site
MYLK_HUMAN do not have hyperphosphorylation site.

Direct Interaction Pair
SourceTargetRelationshipResource
PAK2_HUMANMYLK_HUMANkinase -> substrateRegPhos
ZAGL2_HUMANMYLK_HUMANkinase -> substrateRegPhos
PKN1_HUMANMYLK_HUMANkinase -> substrateHPRD; RegPhos; Robert H Newman (2013)
MYLK_HUMANMYL9_HUMANkinase -> substrateNetPath
MYLK_HUMANML12B_HUMANkinase -> substrateKEGG
ROCK1_HUMANMYLK_HUMANkinase -> substrateKEGG
KCC2G_HUMANMYLK_HUMANkinase -> substrateHPRD
MYLK_HUMANNDF1_HUMANkinase -> substrateRobert H Newman (2013)
CALL6_HUMANMYLK_HUMANotherKEGG

Function Annotation
KEGG Pathway
KEGG IDPathway
hsa04020Calcium signaling pathway
hsa04270Vascular smooth muscle contraction
hsa04510Focal adhesion
hsa04971Gastric acid secretion
hsa04810Regulation of actin cytoskeleton

Gene Ontology
GO IDGO_TermEvidenceOntology
GO:0090303positive regulation of wound healingIDAbiological_process
GO:0004687myosin light chain kinase activityIDAmolecular_function
GO:0005515protein bindingIPImolecular_function
GO:0005829cytosolTAScellular_component
GO:0005886plasma membraneIDAcellular_component
GO:0014820tonic smooth muscle contractionISSbiological_process
GO:0032060bleb assemblyIMPbiological_process
GO:0046872metal ion bindingIEAmolecular_function
GO:0005516calmodulin bindingIEAmolecular_function
GO:0071476cellular hypotonic responseIDAbiological_process
GO:0003779actin bindingIEAmolecular_function
GO:0030027lamellipodiumIDAcellular_component
GO:0004672protein kinase activityIEAmolecular_function
GO:0030335positive regulation of cell migrationIDAbiological_process
GO:0006939smooth muscle contractionISSbiological_process
GO:0032154cleavage furrowIDAcellular_component
GO:0004713protein tyrosine kinase activityIEAmolecular_function
GO:0005524ATP bindingIEAmolecular_function
GO:0006468protein phosphorylationTASbiological_process
GO:0006936muscle contractionTASbiological_process
GO:0060414aorta smooth muscle tissue morphogenesisIMPbiological_process
GO:0004674protein serine/threonine kinase activityIEAmolecular_function
GO:0051928positive regulation of calcium ion transportIDAbiological_process
GO:0001725stress fiberIDAcellular_component
GO:0016772transferase activity, transferring phosphorus-contIEAmolecular_function
GO:0005737cytoplasmIDAcellular_component