Phosphorylation

Protein information

Protein summary

Phosphorylation site

Dephosphorylation site

Mutation site

Differential expressed protein

Hyperphosphorylation site

Direct interaction pair

Function Annotation

Protein Summary

UniProt/SwissProt ID:	RB_HUMAN
Description:	retinoblastoma 1 [Source:HGNC Symbol;Acc:9884]
Location:	chr13 q14.2
Node attribute:	PPI(target); substrate; gene

Phosphorylation site

Position	PhosphoPeptide	Catalytic kinase	Source
S780	RPPTLsPIPHI	P30279(Cyclin D2	RegPhos
Y813	PLKSPyKISEG		HPRD; phosphoELM
T252	NGSPRtPRRGQ	CDC2	RegPhos
T5	RKTA	CDK4	HPRD
S807	GNIYIsPLKSP	CDK2	HPRD
S773	ILQYAsTRPPT	Rb_C	PhosphoSitePlus
Y325	RYEEIyLKNKD		PhosphoSitePlus
T583	DREGPtDHLES		PhosphoSitePlus
T252	NGSPRtPRRGQ		PhosphoSitePlus; phosphoELM
S567	AWLSDsPLFDL	RB_A	PhosphoSitePlus
T823	GLPTPtKMTPR		HPRD; phosphoELM
S788	PHIPRsPYKFP	CDK4	HPRD; RegPhos
S624	TTRVNsTANAE		PhosphoSitePlus
S811	ISPLKsPYKIS	CDC2	RegPhos
T373	VIPPHtPVRTV	CDK_group	phosphoELM; RegPhos
T826	TPTKMtPRSRI		phosphoELM; RegPhos
T821	SEGLPtPTKMT	Rb_C	PhosphoSitePlus
Y813	PLKSPyKISEG	Rb_C	PhosphoSitePlus
T356	FETQRtPRKSN	CDK4	HPRD; RegPhos
T821	SEGLPtPTKMT		phosphoELM
S842	ESFGTsEKFQK	Rb_C	PhosphoSitePlus
S829	KMTPRsRILVS	Rb_C	PhosphoSitePlus
T625	TRVNStANAET		PhosphoSitePlus
S834	SRILVsIGESF	Rb_C	PhosphoSitePlus
T356	FETQRtPRKSN		PhosphoSitePlus; phosphoELM
T373	VIPPHtPVRTV	CDC2	RegPhos
T774	LQYAStRPPTL	Rb_C	PhosphoSitePlus
S37	DPEQDsGPEDL		PhosphoSitePlus; HPRD; phosphoELM; SysPTM
S795	YKFPSsPLRIP		HPRD
Y805	PGGNIyISPLK	Rb_C	PhosphoSitePlus
S249	IPINGsPRTPR	CDC2	RegPhos
S230	YFIKLsPPMLL		PhosphoSitePlus
T823	GLPTPtKMTPR	Rb_C	PhosphoSitePlus
S780	RPPTLsPIPHI	Rb_C	PhosphoSitePlus
S895	HLPGEsKFQQK	Rb_C	PhosphoSitePlus
T252	NGSPRtPRRGQ	CDK1	HPRD
S855	QMVCNsDRVLK		HPRD; phosphoELM; SysPTM
S811	ISPLKsPYKIS	CDK1	HPRD
S811	ISPLKsPYKIS	CDK2	HPRD
S163	LFALFsKLERT	DUF3452	PhosphoSitePlus
T821	SEGLPtPTKMT	CDK2	HPRD; RegPhos
T353	IDSFEtQRTPR		PhosphoSitePlus; HPRD; phosphoELM
S811	ISPLKsPYKIS		phosphoELM
T826	TPTKMtPRSRI	CDK4	HPRD; RegPhos
T140	LKEIDtSTKVD	DUF3452	PhosphoSitePlus
T373	VIPPHtPVRTV	CDK1	HPRD
S811	ISPLKsPYKIS	Rb_C	PhosphoSitePlus
S780	RPPTLsPIPHI	CDK4	HPRD; RegPhos
S807	GNIYIsPLKSP	CDK1	HPRD
S780	RPPTLsPIPHI	CCND2	HPRD
T345	DKTLQtDSIDS		PhosphoSitePlus
S882	FDIEGsDEADG	Rb_C	PhosphoSitePlus
S612	LSPVRsPKKKG	CDK2	HPRD; RegPhos
T826	TPTKMtPRSRI	Rb_C	PhosphoSitePlus
S588	TDHLEsACPLN		PhosphoSitePlus
T252	NGSPRtPRRGQ	CDK_group	phosphoELM; RegPhos
S360	RTPRKsNLDEE		PhosphoSitePlus
S795	YKFPSsPLRIP	Rb_C	PhosphoSitePlus
S855	QMVCNsDRVLK	Rb_C	PhosphoSitePlus; SysPTM
S249	IPINGsPRTPR	CDK1	HPRD
T5	_MPPKtPRKTA		PhosphoSitePlus
T841	GESFGtSEKFQ	Rb_C	PhosphoSitePlus
T241	KEPYKtAVIPI		PhosphoSitePlus
S838	VSIGEsFGTSE	Rb_C	PhosphoSitePlus
S249	IPINGsPRTPR	CDK_group	phosphoELM; RegPhos
Y790	IPRSPyKFPSS	Rb_C	PhosphoSitePlus
T601	LQNNHtAADMY		PhosphoSitePlus; HPRD
T373	VIPPHtPVRTV	RB_A	PhosphoSitePlus
T373	VIPPHtPVRTV		phosphoELM
S807	GNIYIsPLKSP	Rb_C	PhosphoSitePlus
S794	PYKFPsSPLRI	Rb_C	PhosphoSitePlus
Y321	NLSKRyEEIYL		PhosphoSitePlus
S618	PKKKGsTTRVN		PhosphoSitePlus
S807	GNIYIsPLKSP		phosphoELM
S811	ISPLKsPYKIS	CDK_group	phosphoELM; RegPhos
S612	LSPVRsPKKKG		PhosphoSitePlus; phosphoELM
S347	TLQTDsIDSFE		PhosphoSitePlus
S788	PHIPRsPYKFP	Rb_C	PhosphoSitePlus
S230	YFIKLsPPMLL	CDK2	HPRD; RegPhos
T905	KLAEMtSTRTR	Rb_C	PhosphoSitePlus
S807	GNIYIsPLKSP	CDC2	RegPhos
S149	VDNAMsRLLKK	DUF3452	PhosphoSitePlus
S807	GNIYIsPLKSP	CDK_group	phosphoELM; RegPhos
Y606	TAADMyLSPVR		PhosphoSitePlus; HPRD; phosphoELM
S816	SPYKIsEGLPT	Rb_C	PhosphoSitePlus
S350	TDSIDsFETQR		PhosphoSitePlus
S608	ADMYLsPVRSP		PhosphoSitePlus
S249	IPINGsPRTPR		PhosphoSitePlus; phosphoELM
T778	STRPPtLSPIP	Rb_C	PhosphoSitePlus
S780	RPPTLsPIPHI		HPRD
S795	YKFPSsPLRIP	CDK4	HPRD; RegPhos
T5	MPPKTpRKTA	CDK4	RegPhos
S567	AWLSDsPLFDL	CDK2	HPRD; RegPhos
S919	QKMNDsMDTSN	Rb_C	PhosphoSitePlus
S608	ADMYLsPVRSP	CDK2	HPRD; RegPhos

Dephosphorylation site

RB_HUMAN do not have dephosphorylation site.

Mutation site

Ensembl ID	Variation	Source	Cancer name	Pubmed
ENSP00000267163	R320*	TCGA	Ovarian Cancer	21720365, 18772890
ENSP00000267163	R763T	TCGA	Ovarian Cancer	21720365, 18772890
ENSP00000267163	R358G	HPI	Head and Neck Cancer	8776589
ENSP00000267163	E72Q	HPI	Head and Neck Cancer	8605116
ENSP00000267163	R500G	HPI	Head and Neck Cancer	11524739
ENSP00000267163	K530R	HPI	Head and Neck Cancer	7704558
ENSP00000267163	H673P	HPI	Head and Neck Cancer
ENSP00000267163	A635P	HPI	Head and Neck Cancer	7795591
ENSP00000267163	E440*	Ding2008, BIOMART	Lung Cancer	18428421, 18948947, 21930507, 21930502
ENSP00000267163	Q395*	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	R150S	BIOMART	Gastric Cancer	21930507, 21930502
ENSP00000267163	N803K	HPI	Head and Neck Cancer	8605116
ENSP00000267163	L657P	HPI	Head and Neck Cancer	8776589
ENSP00000267163	L662P	HPI	Head and Neck Cancer	10671068
ENSP00000267163	R661W	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	W563*	Ding2008, BIOMART	Lung Cancer	18948947, 21930507, 21930502
ENSP00000267163	Q685P	HPI	Head and Neck Cancer	7795591
ENSP00000267163	R787*	TCGA	Ovarian Cancer	21720365, 18772890
ENSP00000267163	Q702*	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	R661W	OMIM, HPI	Head and Neck Cancer	1352883 10486322, 1352883 7927327 8776589 9311732 18677112 15643604
ENSP00000267163	G310E	HPI	Head and Neck Cancer	9973307
ENSP00000267163	Q217*	Ding2008	Neoplasms by Histologic Type	18428421, 18948947
ENSP00000267163	V654E	HPI	Head and Neck Cancer	7795591
ENSP00000267163	S567L	OMIM, HPI	Head and Neck Cancer	2594029, 2594029 10671068
ENSP00000267163	H549Y	HPI	Head and Neck Cancer	8605116
ENSP00000267163	M457R	HPI	Head and Neck Cancer	8346255
ENSP00000267163	E137D	HPI	Head and Neck Cancer	9311732
ENSP00000267163	R621P	TCGA	Ovarian Cancer	21720365, 18772890
ENSP00000267163	R251*	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	K447Q	HPI	Head and Neck Cancer	9140452
ENSP00000267163	Y659F	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	A264V	TCGA	Ovarian Cancer	21720365, 18772890
ENSP00000267163	R698W	BIOMART	Brain Cancer	21930507, 21930502
ENSP00000267163	Q217*	Ding2008, BIOMART	Lung Cancer	18428421, 18948947, 21930507, 21930502
ENSP00000267163	Y728C	BIOMART	Neoplasms by Histologic Type	21930507, 21930502
ENSP00000267163	K616E	HPI	Head and Neck Cancer	11524739
ENSP00000267163	C712R	OMIM, HPI	Head and Neck Cancer	10486322, 10671068
ENSP00000267163	I185T	HPI	Head and Neck Cancer	7795591
ENSP00000267163	Y424*	TCGA	Neoplasms by Histologic Type	21720365, 18772890
ENSP00000267163	C706Y	HPI	Head and Neck Cancer
ENSP00000267163	R556*	BIOMART	Neoplasms by Histologic Type	21930507, 21930502
ENSP00000267163	E79*	Ding2008, BIOMART	Lung Cancer	18948947, 21930507, 21930502
ENSP00000267163	E440*	Ding2008	Neoplasms by Histologic Type	18428421, 18948947

Differential expressed protein

RB_HUMAN is not differential expressed protein.

Hyperphosphorylation site

Site	Motif	Peptide	Cancer name	Pubmed	Source
S807	Rb_C	PGGNIYIsPLKSPYK	melanoma skin cancer	15502804	PhosphoSitePlus
S811	Rb_C	IYISPLKsPYKISEG	melanoma skin cancer	15502804	PhosphoSitePlus
S795	Rb_C	SPYKFPSsPLRIPGG	melanoma skin cancer	15502804	PhosphoSitePlus

Direct Interaction Pair

Source	Target	Relationship	Resource
ZAGL2_HUMAN	RB_HUMAN	kinase -> substrate	RegPhos
CCND2_HUMAN	RB_HUMAN	kinase -> substrate	HPRD
CDK1_HUMAN	RB_HUMAN	kinase -> substrate	RegPhos
CDK4_HUMAN	RB_HUMAN	kinase -> substrate	RegPhos; NetPath
CDK2_HUMAN	RB_HUMAN	kinase -> substrate	RegPhos; NetPath
CDK6_HUMAN	RB_HUMAN	kinase -> substrate	NetPath
MDM2_HUMAN	RB_HUMAN	other	KEGG
P53_HUMAN	RB_HUMAN	TF -> target gene	TRANSFAC
E2F1_HUMAN	RB_HUMAN	TF -> target gene	TRANSFAC
ATF2_HUMAN	RB_HUMAN	TF -> target gene	TRANSFAC

Function Annotation

KEGG Pathway

KEGG ID	Pathway
hsa05218	Melanoma
hsa05166	HTLV-I infection
hsa05203	Viral carcinogenesis
hsa05212	Pancreatic cancer
hsa05223	Non-small cell lung cancer
hsa05214	Glioma
hsa05169	Epstein-Barr virus infection
hsa05215	Prostate cancer
hsa05200	Pathways in cancer
hsa05220	Chronic myeloid leukemia
hsa05222	Small cell lung cancer
hsa04110	Cell cycle
hsa05219	Bladder cancer

Gene Ontology

GO ID	GO_Term	Evidence	Ontology
GO:0045892	negative regulation of transcription, DNA-dependen	IEA	biological_process
GO:0035189	Rb-E2F complex	TAS	cellular_component
GO:0045892	negative regulation of transcription, DNA-dependen	IDA	biological_process
GO:0030308	negative regulation of cell growth	TAS	biological_process
GO:0051301	cell division	IEA	biological_process
GO:0005667	transcription factor complex	IEA	cellular_component
GO:0005515	protein binding	IEA	molecular_function
GO:0007090	regulation of S phase of mitotic cell cycle	IEA	biological_process
GO:0001047	core promoter binding	IDA	molecular_function
GO:0043550	regulation of lipid kinase activity	IDA	biological_process
GO:0043433	negative regulation of sequence-specific DNA bindi	TAS	biological_process
GO:0003713	transcription coactivator activity	NAS	molecular_function
GO:0007265	Ras protein signal transduction	IEP	biological_process
GO:0003700	sequence-specific DNA binding transcription factor	TAS	molecular_function
GO:0000122	negative regulation of transcription from RNA poly	IEA	biological_process
GO:0045944	positive regulation of transcription from RNA poly	IEA	biological_process
GO:0042551	neuron maturation	IEA	biological_process
GO:0045445	myoblast differentiation	IMP	biological_process
GO:0048565	digestive tract development	IEA	biological_process
GO:0043353	enucleate erythrocyte differentiation	IEA	biological_process
GO:0030521	androgen receptor signaling pathway	NAS	biological_process
GO:0031575	mitotic cell cycle G1/S transition checkpoint	TAS	biological_process
GO:0007346	regulation of mitotic cell cycle	IMP	biological_process
GO:0045842	positive regulation of mitotic metaphase/anaphase	IMP	biological_process
GO:0003677	DNA binding	TAS	molecular_function
GO:0035189	Rb-E2F complex	IEA	cellular_component
GO:0051402	neuron apoptotic process	IEA	biological_process
GO:0031625	ubiquitin protein ligase binding	IPI	molecular_function
GO:0016605	PML body	IDA	cellular_component
GO:0071922	regulation of cohesin localization to chromatin	IMP	biological_process
GO:0019899	enzyme binding	IEA	molecular_function
GO:0016514	SWI/SNF complex	TAS	cellular_component
GO:0045893	positive regulation of transcription, DNA-dependen	NAS	biological_process
GO:0051726	regulation of cell cycle	IEA	biological_process
GO:0005654	nucleoplasm	TAS	cellular_component
GO:0006469	negative regulation of protein kinase activity	IPI	biological_process
GO:0005515	protein binding	IPI	molecular_function
GO:0000084	S phase of mitotic cell cycle	TAS	biological_process
GO:0071930	negative regulation of transcription involved in G	IEA	biological_process
GO:0030182	neuron differentiation	IEA	biological_process
GO:0071459	protein localization to chromosome, centromeric re	IMP	biological_process
GO:0051146	striated muscle cell differentiation	IEA	biological_process
GO:0000785	chromatin	TAS	cellular_component
GO:0000082	G1/S transition of mitotic cell cycle	IEA	biological_process
GO:0031134	sister chromatid biorientation	IMP	biological_process
GO:0051219	phosphoprotein binding	IPI	molecular_function
GO:0007050	cell cycle arrest	IEA	biological_process
GO:0000080	G1 phase of mitotic cell cycle	TAS	biological_process
GO:0006351	transcription, DNA-dependent	IEA	biological_process
GO:0034088	maintenance of mitotic sister chromatid cohesion	IMP	biological_process
GO:0000278	mitotic cell cycle	TAS	biological_process
GO:0008285	negative regulation of cell proliferation	IEA	biological_process
GO:0045879	negative regulation of smoothened signaling pathwa	IEA	biological_process
GO:0005819	spindle	IEA	cellular_component
GO:0000279	M phase	NAS	biological_process
GO:0045651	positive regulation of macrophage differentiation	IEA	biological_process
GO:0090230	regulation of centromere complex assembly	TAS	biological_process
GO:0051318	G1 phase	TAS	biological_process
GO:0000075	cell cycle checkpoint	TAS	biological_process
GO:0005634	nucleus	IDA	cellular_component
GO:0008134	transcription factor binding	IPI	molecular_function
GO:0048667	cell morphogenesis involved in neuron differentiat	IEA	biological_process
GO:0031175	neuron projection development	IEA	biological_process
GO:0019048	virus-host interaction	IEA	biological_process
GO:0007346	regulation of mitotic cell cycle	IEA	biological_process
GO:0050680	negative regulation of epithelial cell proliferati	IEA	biological_process
GO:0050681	androgen receptor binding	NAS	molecular_function
GO:0007050	cell cycle arrest	TAS	biological_process
GO:0006338	chromatin remodeling	TAS	biological_process
GO:0001102	RNA polymerase II activating transcription factor	IEA	molecular_function
GO:0000083	regulation of transcription involved in G1/S phase	TAS	biological_process
GO:0045786	negative regulation of cell cycle	IEA	biological_process
GO:0000082	G1/S transition of mitotic cell cycle	TAS	biological_process
GO:0019900	kinase binding	IDA	molecular_function
GO:0045749	negative regulation of S phase of mitotic cell cyc	TAS	biological_process

Human Phosphorylation Mediated
Signaling Transduction Network

PhoSigNet

Human Phosphorylation Mediated Signaling Transduction Network

PhoSigNet

Human Phosphorylation Mediated
Signaling Transduction Network